| Author | Search for: Ohyama, Kanji1; Search for: Gamborg, Oluf L.1; Search for: Shyluk, Jerry P.1; Search for: Miller, Raymond A.1 |
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| Affiliation | - National Research Council of Canada. The Prairie Regional laboratory
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| Format | Text, Article |
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| Abstract | Uptake of exogenous DNA by plant protoplasts isolated from cultured cells was demonstrated using E. coli ¹⁴C-DNA and Ammi visnaga protoplasts. Between 0.67 to 2.8% of exogenous E. coli ¹⁴C-DNA was taken up into the protoplasts at 28 ᵒC, of which about 20% appeared to be acid-precipitable. The time-course of uptake, the effect of incubation temperature, and the effect of DNA and protoplast concentration were studied. DEAE-dextran, poly-L-lysine, and ply-L-ornithine were found to markedly enhance the uptake. |
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| Publication date | 1973 |
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| Publisher | Institut national de la recherche agronomique |
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| Access condition | - Print copy held at NRC. Digital version available upon request. Contact NRC.NSL-BSN.CNRC@nrc-cnrc.gc.ca
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| Series | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 12952 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 47a72f26-fa7f-49e3-8483-46c56edf578a |
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| Record created | 2023-12-04 |
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| Record modified | 2024-03-11 |
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