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Automatic 1D 1H NMR metabolite quantification for bioreactor monitoring

From National Research Council Canada

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DOIResolve DOI: https://doi.org/10.3390/metabo11030157
AuthorSearch for: ORCID identifier: https://orcid.org/0000-0002-1391-4536; Search for: ORCID identifier: https://orcid.org/0000-0001-8722-4450; Search for: ORCID identifier: https://orcid.org/0000-0002-6039-2355; Search for: 1ORCID identifier: https://orcid.org/0000-0002-9483-8159
Affiliation
  1. National Research Council of Canada. Digital Technologies
FormatText, Article
SubjectNMR; metabolomics; bioreactors; metabolite quantification; quantitative NMR; biomanufacturing; bioprocessing
Abstract
High-throughput metabolomics can be used to optimize cell growth for enhanced production or for monitoring cell health in bioreactors. It has applications in cell and gene therapies, vaccines, biologics, and bioprocessing. NMR metabolomics is a method that allows for fast and reliable experimentation, requires only minimal sample preparation, and can be set up to take online measurements of cell media for bioreactor monitoring. This type of application requires a fully automated metabolite quantification method that can be linked with high-throughput measurements. In this review, we discuss the quantifier requirements in this type of application, the existing methods for NMR metabolomics quantification, and the performance of three existing quantifiers in the context of NMR metabolomics for bioreactor monitoring.
Publication date
PublisherMDPI
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  • Metabolites 11, no. 3.
LanguageEnglish
Peer reviewedYes
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Record identifier49411044-50e0-456b-990e-700e104067d3
Record created2021-06-11
Record modified2021-06-14
Date modified: