| DOI | Resolve DOI: https://doi.org/10.1038/nsmb.1374 |
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| Author | Search for: Tocilj, Ante; Search for: Munger, Christine; Search for: Proteau, Ariane; Search for: Morona, Renato; Search for: Purins, Leanne; Search for: Ajamian, Eunice; Search for: Wagner, John1; Search for: Papadopoulos, Magdalene; Search for: Den Bosch, Luisa Van; Search for: Rubinstein, John L.; Search for: Fethiere, James; Search for: Matte, Allan1; Search for: Cygler, Miroslaw1 |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | Amino Acid Substitution; Bacterial Proteins; biosynthesis; chemistry; crystal structure; Crystallography,X-Ray; enzymology; Escherichia coli; Escherichia coli O157; Escherichia coli Proteins; genetics; Microscopy,Electron,Transmission; Models,Molecular; pharmaceutical; Polysaccharides,Bacterial; Protein; Protein Conformation; Proteins; Salmonella typhimurium; Sequence Deletion |
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| Abstract | The chain length distribution of complex polysaccharides present on the bacterial surface is determined by polysaccharide co-polymerases (PCPs) anchored in the inner membrane. We report crystal structures of the periplasmic domains of three PCPs that impart substantially different chain length distributions to surface polysaccharides. Despite very low sequence similarities, they have a common protomer structure with a long central alpha-helix extending 100 A into the periplasm. The protomers self-assemble into bell-shaped oligomers of variable sizes, with a large internal cavity. Electron microscopy shows that one of the full-length PCPs has a similar organization as that observed in the crystal for its periplasmic domain alone. Functional studies suggest that the top of the PCP oligomers is an important region for determining polysaccharide modal length. These structures provide a detailed view of components of the bacterial polysaccharide assembly machinery |
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| Publication date | 2008 |
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| Language | English |
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| NRC number | NRC-BRI-49529 |
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| NPARC number | 3539594 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 4b89f941-c7a1-4f5c-be24-a64867d42f08 |
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| Record created | 2009-03-01 |
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| Record modified | 2022-11-18 |
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