| DOI | Resolve DOI: https://doi.org/10.1016/0304-4211(77)90051-7 |
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| Author | Search for: Gamborg, O.L.1; Search for: Shyluk, J.P.1; Search for: Brar, D.S.; Search for: Constabel, F.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Abstract | Callus with leafy shoots were obtained from immature embryos of sorghum (Sorghum bicolor L. Moench) collected 12–18 days after pollination. The embryos were cultured on a defined agar medium containing Murashige-Skoog (MS) mineral salts supplemented with 5 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 10–50 μM zeatin. The callus was produced within 10 weeks and further proliferated after subculture on the same medium. Small sections of callus with leafy shoots were transferred onto the same nutrient medium but containing 1–1 μm indoleacetic acid (IAA). Plantlets produced after 8–10 weeks were transferred to pots and grown to maturity. Some plants were sterile while others produced fertile seeds. Meiotic metaphase examination of 25 panicles revealed chromosome number of 2n = 20 corresponding to normal plants with 10-10 anaphase distribution. |
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| Publication date | 1977-09 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | 0304421177900517 |
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| NRC number | NRCC-15967 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 4bf8c994-8604-4e74-afc1-62e6ab594c33 |
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| Record created | 2023-07-06 |
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| Record modified | 2023-07-11 |
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