| Abstract | On-line flow microcalorimetry and fluorometry were applied to the growth of Xanthobacter autotrophicus, a bacterium that is capable of growth on a variety of haloaliphatic compounds as sole carbon sources, and produces a drag-reducing extracellular polysaccharide slime. High correlations were observed between the on-line measurements and the off-line biomass indicators of optical density, cell dry weight, membrane carotenoid pigment extract and the levels of two constitutively produced dehalogenases. Culture fluorescence, in the latter stages of growth, reflected an accumulation of products in the growth medium. The present study demonstrated that on-line monitoring techniques are sufficiently reliable to eliminate the necessity of sample processing, following an initial characterization of the specific microbial process. |
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