| DOI | Resolve DOI: https://doi.org/10.1007/978-1-0716-0795-4_31 |
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| Author | Search for: Akache, Bassel1; Search for: Stark, Felicity C.1; Search for: McCluskie, Michael J.1 |
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| Affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Book Chapter |
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| Subject | vaccines; immunoglobulin; antibody; humoral response; ELISA |
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| Abstract | Direct ELISA allows for the measurement of antibody levels to a particular antigen. Serum or plasma from the vaccinated subject are incubated on high-binding capacity microplates precoated with the antigen of interest and detected utilizing an enzyme-linked secondary antibody. Herein, using influenza hemagglutinin as model antigen, we describe the quantification of antigen-specific IgG titers in mouse serum to measure vaccine-induced humoral responses. |
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| Publication date | 2020-09-22 |
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| Publisher | Humana |
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| In | |
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| Series | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-HHT_53441 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 4c4af990-50e6-434f-b13b-04406e361d65 |
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| Record created | 2021-01-08 |
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| Record modified | 2021-01-09 |
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