| DOI | Resolve DOI: https://doi.org/10.1002/pmic.200600112 |
|---|
| Author | Search for: Melanson, Jeremy E.1; Search for: Avery, Steven L.1; Search for: Pinto, Devanand M.1 |
|---|
| Affiliation | - National Research Council of Canada. NRC Institute for Marine Biosciences
|
|---|
| Format | Text, Article |
|---|
| Subject | isotopic labeling; quantitative proteomics |
|---|
| Abstract | Peptide dimethylation with isotopically coded formaldehydes was evaluated as a potential alternative to techniques such as the iTRAQ method for comparative proteomics. The isotopic labeling strategy and custom-designed protein quantitation software were tested using protein standards and then applied to measure proteins levels associated with Alzheimer's disease (AD). The method provided high accuracy (10% error), precision (14% RSD) and coverage (70%) when applied to the analysis of a standard solution of BSA by LC-MS/MS. The technique was then applied to measure protein abundance levels in brain tissue afflicted with AD relative to normal brain tissue. 2-D LC-MS analysis identified 548 unique proteins (p <0.05). Of these, 349 were quantified with two or more peptides that met the statistical criteria used in this study. Several classes of proteins exhibited significant changes in abundance. For example, elevated levels of antioxidant proteins and decreased levels of mitochondrial electron transport proteins were observed. The results demonstrate the utility of the labeling method for high-throughput quantitative analysis. |
|---|
| Publication date | 2006-08-08 |
|---|
| Terms of use | - © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
|
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NRC number | NRCC 55861
1573 |
|---|
| NPARC number | 3538152 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 4c516310-7f17-40fa-b762-2f322864169b |
|---|
| Record created | 2009-03-01 |
|---|
| Record modified | 2020-04-22 |
|---|
