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Genetic characterization of Escherichia coli O157:H7 strains isolated from the one-humped camel (Camelus dromedarius) by using microarray DNA technology

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/s12033-011-9466-7
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Affiliation
  1. National Research Council of Canada. NRC Biotechnology Research Institute
FormatText, Article
Subjectanimal disease; classification; DNA microarray; Escherichia coli infections; Escherichia coli O157; genetics; isolation and purification; methodology; microbiology; camels; DNA, bacterial; feces; genes, bacterial; oligonucleotide array sequence analysis; virulence factors; Escherichia coli
Abstract
From the Camelidae family members, several serotypes of Escherichia coli (E. coli) have recently been isolated from diarrhoeic and non-diarrhoeic faecal samples. To date Shiga toxin-producing E. coli (STEC) strains have never been typed in one-humped camel (Camelus dromedarius). In the present study, two E. coli O157:H7 strains isolated from sick dromedaries were investigated. Virulence gene profiles were determined using a custom E. coli virulence DNA microarray, composed of 70-mer oligonucleotide probes targeting 264 virulence or related genes of known E. coli pathotypes. Both strains displayed positive hybridization signals for the Locus of enterocyte effacement (LEE) gene probes (ler, eae, espA, espB, tir genes), two Shiga toxin probes (stx1 and stx2), the O157 O-antigen specific probe, various virulence plasmid (pO157) probes like katP in addition to other accessory virulence genes characterized in STEC.
Publication date
In
  • Molecular Biotechnology 51, no. 3 (2012): 283–288.
LanguageEnglish
Peer reviewedYes
NPARC number21270863
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Record identifier4d30fb53-d158-4ad1-be72-48771b0fd1ba
Record created2014-02-17
Record modified2022-11-18
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