| Author | Search for: Holl, F. B.1 |
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| Affiliation | - National Research Council of Canada. The Prairie Regional laboratory
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| Format | Text, Article |
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| Abstract | A rapid expansion has recently taken place in research into methods of direct transfer of heritable information from microorganisms and plants into the somatic cells of other plant species using treatment with exogenous DNA. The DNAse activity of the host material may play a role in determining the ultimate fate of the fed DNA. The occurrence and distribution of DNAse in seeds, seedings, suspension culture cells and protoplasts of the soybean Glycine max L. var. Mandarin are described in this communication. Increased levels of enzyme activity are associated with cells and tissues which are dividing such as root meristem and rapidly dividing cells in suspension culture. In contrast, low levels of DNAse activity were detected in cotyledons and freshly isolated protoplasts which were undergoing little or no cellular division. DNAse activity was inhibited by DEAE-dextran5'-AMP and protamine sulphate. The possible effectiveness of such compounds in assisting the uptake of intact DNA is considered. The use of DNAse in soybean as an indicator of cellular receptivity to added DNA is proposed. |
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| Publication date | 1973 |
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| Publisher | Institut national de la recherche agronomique |
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| Access condition | - Print copy held at NRC. Digital version available upon request. Contact NRC.NSL-BSN.CNRC@nrc-cnrc.gc.ca
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| Series | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 12948 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 4df0375b-5644-4591-830a-6302ca350e3a |
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| Record created | 2023-12-12 |
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| Record modified | 2024-03-11 |
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