| Download | - View accepted manuscript: MAM gene silencing leads to the induction of C3 and reduction of C4 and C5 side chain aliphatic glucosinolates in Brassica napus (PDF, 657 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/s11032-010-9444-y |
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| Author | Search for: Liu, Zheng; Search for: Hammerlindl, Joe1; Search for: Keller, Wilf; Search for: McVetty, Peter B. E.; Search for: Daayf, Fouad; Search for: Quiros, Carlos F.; Search for: Li, Genyi |
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| Affiliation | - National Research Council of Canada. NRC Plant Biotechnology Institute
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| Format | Text, Article |
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| Subject | Brassica napus; Glucosinolate; Gene silencing |
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| Abstract | Methylthioalkylmalate (MAM) synthases and their associated genes that have been extensively investigated in Arabidopsis control the side-chain elongation of methionine during the synthesis of aliphatic glucosinolates. A Brassica homolog of the Arabidopsis MAM genes was used in this study to analyze the role of MAM genes in B. napus through RNA interference (RNAi). The silencing of the MAM gene family in B. napus canola and B. napus rapeseed resulted in the reduction of aliphatic glucosinolates and total glucosinolate content. The results indicated that RNAi has potential for reducing glucosinolate content and improving meal quality in B. napus canola and rapeseed cultivars. Interestingly, MAM gene silencing in B. napus significantly induced the production of 2-propenyl glucosinolate, a 3-carbon side-chain glucosinolate commonly found in B. juncea mustard. Most transgenic plants displayed induction of 2-propenyl glucosinolate; however, the absolute content of this glucosinolate in transgenic B. napus canola was relatively low (less than 1.00 μmol g−1 seed). In the high glucosinolate content progenies derived from the crosses of B. napus rapeseed and transgenic B. napus canola, MAM gene silencing strongly induced the production of 2-propenyl glucosinolate to high levels (up to 4.45 μmol g−1 seed). |
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| Publication date | 2011-04 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 54646 |
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| NPARC number | 19727318 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 4e7d2151-2c4f-4815-ab55-7fea31fbed5d |
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| Record created | 2012-04-17 |
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| Record modified | 2020-04-21 |
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