| DOI | Resolve DOI: https://doi.org/10.1504/IJNBM.2012.051704 |
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| Author | Search for: Kell, Arnold J.1; Search for: Barnes, Michael L.1; Search for: Slater, Kathryn; Search for: Lavigne, Carole |
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| Affiliation | - National Research Council of Canada. Security and Disruptive Technologies
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| Format | Text, Article |
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| Subject | Cellular uptake; CXCR4; Dye-doped silica nanoparticles; HIV-1 co-receptor; Serum-containing medium; siRNA delivery; Drug therapy; Genetic engineering; Nanoparticles; Nanostructured materials; chemokine receptor CXCR4; messenger RNA; nanoparticle; nuclease; polyethyleneimine; silicon dioxide; small interfering RNA; tetramethylrhodamine doped silica nanoparticle; cell viability; cellular distribution; chemical binding; endosome; enzyme degradation; gene silencing; human cell; Human immunodeficiency virus 1; lysosome; mammal cell; nonviral gene delivery system; protein expression; RNA binding; RNA degradation |
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| Abstract | The development of non-viral DNA delivery systems using nanomaterials has attracted much research interest for its potential in biomedicine. However, for these new nanocarriers to be successfully used in therapeutic applications they have to overcome many barriers. Here, we report the development and characterisation of polyethyleneimine-modified tetramethylrhodamine-doped silica nanoparticles as a vehicle to deliver siRNA in the presence of serum. We have demonstrated that polyethyleneiminemodified tetramethylrhodamine-doped silica nanoparticles bind and protect siRNA against nuclease degradation and facilitate cellular uptake and intracellular delivery of the siRNA in HeLa-derived TZM-bl cells. The nanoparticles can penetrate TMZ-bl cells at concentrations as low as 1 μg/mL and can escape the lysosomal and endosomal cavities. Following delivery, the nanoparticles release active siRNA targeting the co-receptor CXCR4 for HIV-1 to achieve reduction in the targeted mRNA and protein expression. These nanoparticles are also non-toxic for the cells and are capable of carrying out all of these functions in the presence of serum, a characteristic that is critical if such nanoparticles are to be employed in any type of in vivo application. Copyright © 2012 Inderscience Enterprises Ltd. |
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| Publication date | 2012 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21270095 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 4e9c3ab9-02ce-4980-b6bf-5f13d7a3ad09 |
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| Record created | 2013-12-23 |
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| Record modified | 2020-04-21 |
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