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Far-red fluorescent genetically encoded calcium ion indicators

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DOIResolve DOI: https://doi.org/10.1038/s41467-025-58485-z
AuthorSearch for: ; Search for: ORCID identifier: https://orcid.org/0000-0002-1138-3217; Search for: ; Search for: ORCID identifier: https://orcid.org/0000-0002-6336-566X; Search for: ; Search for: ORCID identifier: https://orcid.org/0000-0001-9072-1300; Search for: ORCID identifier: https://orcid.org/0000-0001-7891-2462; Search for: ; Search for: ORCID identifier: https://orcid.org/0000-0001-8131-1772; Search for: ORCID identifier: https://orcid.org/0000-0002-1247-6087; Search for: ORCID identifier: https://orcid.org/0009-0009-7638-9538; Search for: ORCID identifier: https://orcid.org/0000-0002-0096-7321; Search for: ; Search for: ; Search for: ; Search for: 1ORCID identifier: https://orcid.org/0000-0002-1788-7969; Search for: ORCID identifier: https://orcid.org/0000-0003-2406-6728; Search for: ORCID identifier: https://orcid.org/0000-0002-0374-2005; Search for: ORCID identifier: https://orcid.org/0000-0003-0788-5617; Search for: ORCID identifier: https://orcid.org/0000-0002-2864-7469; Search for: ORCID identifier: https://orcid.org/0000-0002-8430-070X; Search for: ORCID identifier: https://orcid.org/0000-0002-3425-3589; Search for: ; Search for: ORCID identifier: https://orcid.org/0000-0002-9330-2934; Search for: ORCID identifier: https://orcid.org/0000-0002-8699-2404; Search for: ORCID identifier: https://orcid.org/0000-0003-4117-0279; Search for: ORCID identifier: https://orcid.org/0000-0003-0604-092X
Affiliation
  1. National Research Council Canada. Medical Devices
FunderSearch for: U.S. Department of Health & Human Services | National Institutes of Health; Search for: Canadian Institutes of Health Research; Search for: Natural Sciences and Engineering Research Council of Canada; Search for: MEXT | Japan Society for the Promotion of Science
FormatText, Article
Abstract
Genetically encoded calcium ion (Ca²⁺) indicators (GECIs) are widely-used molecular tools for functional imaging of Ca²⁺ dynamics and neuronal activities with single-cell resolution. Here we report the design and development of two far-red fluorescent GECIs, FR-GECO1a and FR-GECO1c, based on the monomeric far-red fluorescent proteins mKelly1 and mKelly2. FR-GECOs have excitation and emission maxima at ~596 nm and ~644 nm, respectively, display large responses to Ca²⁺ in vitro (ΔF/F₀ = 6 for FR-GECO1a, 18 for FR-GECO1c), are bright under both one-photon and two-photon illumination, and have high affinities (apparent Kd = 29 nM for FR-GECO1a, 83 nM for FR-GECO1c) for Ca²⁺. FR-GECOs offer sensitive and fast detection of single action potentials in neurons, and enable in vivo all-optical manipulation and measurement of cellular activities in combination with optogenetic actuators.
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PublisherSpringer Nature
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  • Nature Communications 16, no. 1, 3318 (7 April 2025): 1–14.
LanguageEnglish
Peer reviewedYes
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Record identifier4fc0bddd-9f5b-4511-add3-95a398b2ae82
Record created2026-02-27
Record modified2026-02-27

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