Language selection

Government of Canada / Gouvernement du Canada

Search

Single-step 3D bioprinting of alginate–collagen type I hydrogel fiber rings to promote angiogenic network formation

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1089/ten.tec.2024.0083
AuthorSearch for: 1, 2ORCID identifier: https://orcid.org/0000-0002-1560-8866; Search for: 1; Search for: 1; Search for: 3ORCID identifier: https://orcid.org/0000-0002-7179-8588; Search for: 1; Search for: 4; Search for: 5; Search for: 1, 3
Affiliation
  1. National Research Council of Canada. Human Health Therapeutics
  2. Carleton University. Department of Systems and Computer Engineering
  3. University of Ottawa. Department of Chemical and Biological Engineering
  4. Aspect Biosystems Ltd.
  5. University of Ottawa, Ottawa. Department of Chemical and Biological Engineering
FormatText, Article
Abstract
In the advent of tissue engineering and regenerative medicine, the demand for innovative approaches to biofabricate complex vascular structures is increasing. We describe a single-step 3D bioprinting method leveraging Aspect Biosystems RX1 technology, which integrates the crosslinking step at a flow-focusing junction, to biofabricate immortalized adult rat brain endothelial cell (SV-ARBEC)-encapsulated alginate–collagen type I hydrogel rings. This single-step biofabrication process involves the strategic layer-by-layer assembly of hydrogel rings, encapsulating SV-ARBECs in a spatially controlled manner while optimizing access to media and nutrients. The spatial arrangement of the SV-ARBECs within the rings promotes spontaneous angiogenic network formation and the constrained deposition of cells within the hydrogel matrix facilitates tissue-like organized vascular-like network development. This approach provides a platform that can be adapted to many different endothelial cell types and leveraged to better understand the mechanisms driving angiogenesis and vascular-network formation in 3D bioprinted constructs supporting the development of more complex tissue and disease models for advancing drug discovery, tissue engineering, and regenerative medicine applications.
Publication date
PublisherMary Ann Liebert
In
  • Tissue Engineering Part C: Methods 30, no. 7 (17 July 2024): 289–306.
LanguageEnglish
Peer reviewedYes
Export citationExport as RIS
Report a correctionReport a correction (opens in a new tab)
Record identifier50a8f325-20fc-49d0-8c5c-81734b5e7628
Record created2024-09-13
Record modified2024-09-13
Date modified: