| DOI | Resolve DOI: https://doi.org/10.1361/cp2009itsc0366 |
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| Author | Search for: Lima, R. S1; Search for: Dimitrievska, S.1; Search for: Bureau, M. N.; Search for: Marple, B. R.1; Search for: Petit, A.; Search for: Mwale, F.; Search for: Antoniou, J. |
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| Affiliation | - National Research Council Canada. NRC Industrial Materials Institute
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| Format | Text, Article |
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| Conference | Expanding Thermal Spray Performance to New Markets and Applications: 2009 International Thermal Spray Conference, ITSC 2009, May 4-7, 2009, Las Vegas, NV, USA |
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| Abstract | Biomedical thermal spray coatings produced via high velocity oxy-fuel (HVOF) from nanostructured titania (n-TiO2) and 10wt% hydroxyapatite (HA) (n-TiO2-10wt%HA) powders have been engineered as possible future alternatives to HA coatings deposited via air plasma spray (APS). This approach was chosen due to (i) the stability of TiO2 in the human body (i.e., no dissolution) and (ii) bond strength values on Ti-6Al4V substrates more than two times higher than those of APS HA coatings. To explore the bioperformance of these novel materials and coatings, human mesenchymal stem cells (hMSCs) were cultured from 1 to 21 days on the surface of HVOF-sprayed n-TiO2 and n-TiO2-10wt%HA coatings. APS HA coatings and uncoated Ti-6Al-4V substrates were employed as controls. The active profiles of the hMSCs were evaluated for (i) cell proliferation by Alamar Bleu assay, (ii) biochemical analysis of alkaline phosphatase (ALP) activity, (iii) cytoskeleton organization (fluorescent/confocal microscopy) and (iv) cell/substrate interaction via scanning electron microscopy (SEM). Cell proliferation and biochemical analysis indicated that the hMSCs cultured on nTiO2-10wt%HA coatings exhibited similar or superior levels of bioactivity to hMSC cultured on APS HA. The cytoskeleton organization demonstrated a higher degree of cell proliferation and attachment on the HVOF-sprayed nTiO2-10wt%HA coatings. These results are considered promising for engineering improved performance and increased longevity in the next generation of thermally sprayed biomedical coatings. |
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| Publication date | 2009-05 |
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| Publisher | ASM International |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23004805 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 53a25180-649f-428a-b76e-e3f0f79a4649 |
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| Record created | 2018-12-20 |
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| Record modified | 2020-04-16 |
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