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In vitro hepatic biotransformation of the algal toxin pectenotoxin-2

From National Research Council Canada

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DOIResolve DOI: https://doi.org/10.1016/j.toxcx.2020.100031
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Affiliation
  1. National Research Council of Canada. Metrology Research Centre
FormatText, Article
Subjectacid; oxygen; pectenotoxin 1; pectenotoxin 11; pectenotoxin 13; pectenotoxin 2; seco acid; toxin; unclassified drug; aerobic metabolism; animal cell; atom; biotransformation; cell suspension; controlled study; hydrolysis; in vitro study; liquid chromatography-mass spectrometry; liver cell; liver metabolism; male; metabolite; nonhuman; priority journal; rat; retention time; Wistar rat
Abstract
We have investigated the in vitro metabolism of pectenotoxin-2 (PTX-2) using primary hepatocytes from Wistar rats in suspension. Purified PTX-2 was rapidly metabolized. Two major and several minor oxidized PTX-2 metabolites were formed, none of which had retention times corresponding to PTX-1, -11, or −13. Hydrolysis products, such as PTX-2 seco acid, were not observed. Preliminary multi-stage LC-MS analyses indicated that the major hepatic PTX-2 metabolites resulted from the insertion of an oxygen atom at the positions C-19 to C-24, or at C-44. The rapid oxidative metabolism may explain the low oral toxicity of PTXs observed in vivo studies.
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PublisherElsevier
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  • Toxicon: X 6, 100031.
LanguageEnglish
Peer reviewedYes
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Record identifier546afb45-99fe-486d-a38c-0fe7de9158be
Record created2020-06-19
Record modified2020-07-22
Date modified: