In vitro hepatic biotransformation of the algal toxin pectenotoxin-2

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DOI	Resolve DOI: https://doi.org/10.1016/j.toxcx.2020.100031
Author	Search for: Sandvik, Morten; Search for: Miles, Christopher O.¹; Search for: Wilkins, Alistair L.; Search for: Fæste, Christiane
Affiliation	National Research Council Canada. Metrology Research Centre
Format	Text, Article
Subject	acid; oxygen; pectenotoxin 1; pectenotoxin 11; pectenotoxin 13; pectenotoxin 2; seco acid; toxin; unclassified drug; aerobic metabolism; animal cell; atom; biotransformation; cell suspension; controlled study; hydrolysis; in vitro study; liquid chromatography-mass spectrometry; liver cell; liver metabolism; male; metabolite; nonhuman; priority journal; rat; retention time; Wistar rat
Abstract	We have investigated the in vitro metabolism of pectenotoxin-2 (PTX-2) using primary hepatocytes from Wistar rats in suspension. Purified PTX-2 was rapidly metabolized. Two major and several minor oxidized PTX-2 metabolites were formed, none of which had retention times corresponding to PTX-1, -11, or −13. Hydrolysis products, such as PTX-2 seco acid, were not observed. Preliminary multi-stage LC-MS analyses indicated that the major hepatic PTX-2 metabolites resulted from the insertion of an oxygen atom at the positions C-19 to C-24, or at C-44. The rapid oxidative metabolism may explain the low oral toxicity of PTXs observed in vivo studies.
Publication date	2020-03-23
Publisher	Elsevier
In	Toxicon: X 6, 100031.
Language	English
Peer reviewed	Yes
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Record identifier	546afb45-99fe-486d-a38c-0fe7de9158be
Record created	2020-06-19
Record modified	2020-07-22

Date modified:: 2026-01-23