| Download | - View accepted manuscript: Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy (PDF, 788 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1117/1.3490641 |
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| Author | Search for: Slepkov, Aaron D.1; Search for: Ridsdale, Andrew1; Search for: Wan, Huei-Ning; Search for: Wang, Ming-Hao; Search for: Pegoraro, Adrian F.1; Search for: Moffatt, Doug1; Search for: Pezacki, John P.1; Search for: Kao, Fu-Jen; Search for: Stolow, Albert1 |
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| Affiliation | - National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
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| Format | Text, Article |
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| Subject | Coherent anti-Stokes Raman scattering; CARS Microscopy |
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| Abstract | We demonstrate the simultaneous collection and separation of femtosecond-laser-based forward-collected coherent anti-Stokes Raman scattering (F-CARS) and two-photon-excitation-induced fluorescence lifetime images (FLIM) using time-correlated single photon counting (TCSPC). We achieve this in a nondescanned geometry using a single multimode fiber without significant loss of light, field of view, and most importantly, TCSPC timing fidelity. In addition to showing the ability to separate CARS images from FLIM images using time gating, we also demonstrate composite multimodal epicollected FLIM imaging with fiber-collected F-CARS imaging in live cells. |
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| Publication date | 2011-02-01 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 19739656 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 55562410-d97e-49a5-9ca3-a785df2ffbc4 |
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| Record created | 2012-04-02 |
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| Record modified | 2020-04-21 |
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