| DOI | Resolve DOI: https://doi.org/10.1007/978-1-0716-3666-4_5 |
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| Author | Search for: Baardsnes, Jason1ORCID identifier: https://orcid.org/0000-0001-5587-3034; Search for: Paul-Roc, Béatrice1 |
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| Affiliation | - National Research Council Canada. Human Health Therapeutics
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| Format | Text, Book Chapter |
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| Abstract | Surface plasmon resonance (SPR) allows for the label-free determination of the binding affinity and rate constants of bimolecular interactions. Here, we describe the method used for the analysis of the Ace2–SARS-CoV2 S-protein interaction using indirect capture of the S-protein onto the SPR surface, and flowing monomeric Ace2. This method will allow for the determination of the rate constants for affinity, with additional analysis that is achievable using S-protein capture levels in conjunction with the sensorgram response for relative activity benchmarking. |
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| Publication date | 2024-02-06 |
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| Publisher | Springer |
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| In | |
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| Series | |
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| Note | Erratum published July 16, 2024. DOI: 10.1007/978-1-0716-3666-4_21 |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 57cf5488-8433-49c9-80f7-81c9098f6018 |
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| Record created | 2024-11-04 |
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| Record modified | 2024-12-11 |
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