DOI | Resolve DOI: https://doi.org/10.1007/BF01146776 |
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Author | Search for: Hong, H. P.1; Search for: Gerster, J. L.; Search for: Datla, R. S. S.1; Search for: Albani, D.; Search for: Scoles, G.; Search for: Keller, W.1; Search for: Robert, L. S. |
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Affiliation | - National Research Council of Canada. NRC Plant Biotechnology Institute
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Format | Text, Article |
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Subject | pollen; polygalacturonase; promoter; Brassica; transformation; GUS |
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Abstract | A 647-bp 5′-flanking fragment obtained from genomic clone Sta 44G(2) belonging to a family of polygalacturonase genes expressed inBrassica napus pollen was fused to the β-glucuronidase (GUS) marker gene. This fusion construct was introduced intoB. napus plants viaAgrobacterium tumefaciens transformation. Analysis of the transgenic B. napus plants revealed that this promoter fragment is sufficient to direct GUS expression specifically in the anther and that GUS activity increases in pollen during maturation. |
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Publication date | 1997-03 |
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Publisher | Springer Link |
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In | |
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Language | English |
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Peer reviewed | Yes |
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Identifier | BF01146776 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | 59009f07-c783-41c5-bfd5-715f4654e37a |
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Record created | 2023-11-01 |
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Record modified | 2023-11-01 |
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