| DOI | Resolve DOI: https://doi.org/10.1139/W2012-022 |
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| Author | Search for: MacLean, L.L.1; Search for: Vinogradov, E.1; Search for: Pagotto, F.; Search for: Perry, M.B.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | 3 deoxy manno octulosonic acid; 3 hydroxybutyric acid; 3,6 dideoxy 3 3 hydroxybutyramido dextro glucose; bacterium lipopolysaccharide; glucose derivative; n acetylglucosamine; O antigen; pentasaccharide; polymer; rhamnose; unclassified drug; antigen; bacterium; nuclear magnetic resonance; pathogen; polymer; polysaccharide; antigen structure; bacterial growth; bacterium isolation; carbohydrate analysis; Cronobacter dublinensis; hydrolysis; molecular weight; nuclear magnetic resonance; peroxidation; Acetylglucosamine; Amino Acid Sequence; Carbohydrate Sequence; Cronobacter; Glucose; Hydrolysis; Lipopolysaccharides; Magnetic Resonance Spectroscopy; O Antigens; Oligosaccharides; Bacteria (microorganisms); Enterobacter sakazakii; Negibacteria |
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| Abstract | Cronobacter dublinensis (formerly Enterobacter sakazakii) HPB 3169 is a pathogenic Gram-negative bacterium that produces a smooth-type lipopolysaccharide in which the antigenic O-polysaccharide component was determined to be a repeating pentasaccharide unit composed of L-rhamnose; 2-acetamido-2-deoxy-D-glucose; 3,6-dideoxy-3-(R)-3-hydroxybutyramido-Dglucose; and 3-deoxy-manno-oct-2-ulosonic acid in the respective molar ratio 2:1:1:1. Chemical and 2D NMR analyses of the O-polysaccharide and a pentasaccharide derived by the mild acid hydrolysis of the ketosyl linkage of the Kdo (3-deoxy-D-manno-2-octulosonic acid) residue in the O-polysaccharide established that the O-antigen is a high molecular mass unbranched polymer of a repeating pentasaccharide unit and has the structure where Bu is a (R)-3-hydroxybutanoyl substituent. The O-antigen is structurally similar to that of the recently reported Cronobacter sakazakii strain G706 (designated as serotype O5), except that in strain G706 the D-Qui3N is in its N-acetyl form, in contrast to its presence as a 3-deoxy-3-(R)-3-hydroxybutyramido derivative in the C. sakazakii HPB 3169 strain O-antigen. |
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| Publication date | 2012 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21269295 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 592b7849-d4ca-444b-a261-f0b199aefac1 |
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| Record created | 2013-12-12 |
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| Record modified | 2020-04-21 |
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