| DOI | Resolve DOI: https://doi.org/10.1086/336875 |
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| Author | Search for: Gamborg, O. L.1; Search for: Shyluk, J. P.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Abstract | Morphogenesis in flax (Linum usitatissimum L `Redwing') was studied in hypocotyl sections taken 3-5 days after removal of the cotyledons and shoots and the sections were cultured on a Murashige and Skoog (1962) medium Zeatin and other cytokinins at 1-10 μM enhanced bud initiation and shoot development on the hypocotyl explants. Complete plants were obtained by culturing shoot tips in B5 medium at half strength and with 1 μM indoleacetic acid Plants were also obtained from callus derived from the hypocotyls. Methods for isolation of protoplasts from hypocotyls are described. When cultured, the protoplasts regenerated cell walls, divided, and formed callus. The callus differentiated with the formation of roots. |
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| Publication date | 1976-12 |
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| Publisher | University of Chicago Press Journals |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 15409 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 5994edff-9394-40ea-8d22-58997ae528cc |
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| Record created | 2023-07-27 |
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| Record modified | 2023-07-27 |
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