| DOI | Resolve DOI: https://doi.org/10.4172/2157-2526.1000119 |
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| Author | Search for: Geissler, Matthias1; Search for: Isabel, Sandra; Search for: Voisin, Benoît2; Search for: Fauvel, Chantal; Search for: Boissinot, Maurice; Search for: Bergeron, Michel G.; Search for: Veres, Teodor1 |
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| Affiliation | - National Research Council of Canada. Medical Devices
- National Research Council of Canada
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| Format | Text, Article |
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| Subject | Bacillus spores; sonication; spore lysis; PCR; Sample transfer |
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| Abstract | This paper describes the design, functioning and use of an ultrasonic modular system intended for rapid extraction and fragmentation of DNA from microbial organisms following sample collection in the field. PCR assessment of the DNA extracts revealed that the system can disrupt Bacillus atrophaeus spores, a simulant for Bacillus anthracis, in less than 1 min, providing a DNA yield equivalent to that of a commercial nucleic acid extraction method. Simulation of the transfer from a contaminated to a secure area confirmed that the sample remained confined within the module while the exterior surface can be decontaminated through immersion in a disinfectant solution. |
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| Publication date | 2012-11-10 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 140326 |
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| NPARC number | 21268115 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 5bd60e26-548a-4bbf-9ae2-dda503318ab6 |
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| Record created | 2013-04-12 |
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| Record modified | 2020-04-21 |
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