| Download | - View final version: Delivery of siRNA using cationic rosette nanotubes for gene silencing (PDF, 4.4 MiB)
- View supplementary information: Delivery of siRNA using cationic rosette nanotubes for gene silencing (PDF, 2.2 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1039/D3BM01115A |
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| Author | Search for: Ho, Uyen1; Search for: El-Bakkari, Mounir1; Search for: Alshamsan, Aws1; Search for: Cho, Jae-Young1; Search for: Yamazaki, Takeshi1; Search for: Hemraz, Usha D.2ORCID identifier: https://orcid.org/0000-0001-5151-9484; Search for: Fenniri, Hicham3 |
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| Affiliation | - National Research Council of Canada. Nanotechnology
- National Research Council of Canada. Human Health Therapeutics
- National Research Council of Canada. Security and Disruptive Technologies
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| Funder | Search for: National Research Council Canada; Search for: Government of Canada; Search for: Natural Sciences and Engineering Research Council of Canada; Search for: University of Alberta |
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| Format | Text, Article |
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| Abstract | The quest for new therapeutic treatments for hereditary diseases has led to many advances in RNA interference (RNAi) and gene silencing. While this technique has the potential to address many problems, the key to its continued use is the development of effective delivery strategies that would reduce cellular toxicity and increase silencing efficiency. Rosette nanotubes (RNTs) are biomimetic supramolecular nanostructures formed through the self-assembly of hybrid guanine–cytosine (G∧C) DNA bases. Here, we used bioactive RNTs for siRNA delivery and gene silencing. Fifteen lysine-functionalized twin-G∧C motifs (KnT, n = 1 to 15) were synthesized using solid phase peptide synthesis to produce building blocks that self-assembled to produce cationic RNTs under physiological conditions. The intracellular uptake of siRNA delivered by the oligo-L-lysine RNTs was examined and it was found that the complexation of siRNA was affected by the cationic charges from the lysine residues and the length of RNTs formed, with the higher charged KnT RNTs delivering siRNA to the cells at a faster rate. In addition, by protecting siRNA from serum degradation, KnT RNTs were shown to deliver their cargo to the cells effectively via the endocytic pathway. A reduction in the expression (∼70%) of the target stat3 protein was observed during gene expression analysis in HCT116 and A549 cell lines. |
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| Publication date | 2023-09-21 |
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| Publisher | Royal Society of Chemistry |
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| Licence | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 5c165d48-d0a4-4114-8301-ab0b3544d82e |
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| Record created | 2024-04-18 |
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| Record modified | 2024-04-18 |
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