| DOI | Resolve DOI: https://doi.org/10.1016/S0044-328X(76)80167-5 |
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| Author | Search for: Weber, Gerd; Search for: Constabel, Friedrich1; Search for: Williamson, Francis; Search for: Fowke, Lawrence; Search for: Gamborg, Oluf L.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Subject | protoplasts; PEG-induced fusion; frequency of hetekaryocytes; Pisum; Vicia |
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| Abstract | Protoplasts from pea leaves (Pisum sativum) and vetch cell suspension cultures (Vicia hajastana) were fused by agglutination with polyethylene glycol (PEG 1540). The frequency of heterokaryocytes was a function of the duration of preincubation, i.e. the time lapsed between the removal of cell wall digesting enzymes from the isolated protoplasts and agglutination with PEG. The yield of heterokaryocytes decreased from 9 % after 5 min to 1 % after 2 h of preincubation. The most obvious drop occurred between 5 and 15 min of preincubation. The decrease in fusion frequency may be due to rapid cell wall regeneration in vetch protoplasts. |
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| Publication date | 1976-09 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | S0044328X76801675 |
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| NRC number | NRCC 15404 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 5d8b00b4-2d89-4a74-b6fa-92e36d261329 |
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| Record created | 2023-07-26 |
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| Record modified | 2023-07-26 |
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