| DOI | Resolve DOI: https://doi.org/10.1371/journal.pone.0078315 |
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| Author | Search for: Liu, J.1; Search for: Guo, L.; Search for: Yin, F.1; Search for: Zhang, Y.; Search for: Liu, Z.; Search for: Wang, Y.1 |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
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| Format | Text, Article |
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| Subject | 2 oxoglutaric acid; adenosine triphosphate; geniposide; glucose; pyruvate carboxylase; animal cell; cell protection; diabetes mellitus; enzyme activation; enzyme regulation; enzyme synthesis; gluconeogenesis; glucose metabolism; glucose transport; immunoblotting; insulin release; nucleotide sequence; pancreas islet beta cell; protein analysis; protein blood level; protein expression; protein function; rat |
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| Abstract | Glucose-stimulated insulin secretion (GSIS) is essential to the control of metabolic fuel homeostasis. The impairment of GSIS is a key element of β-cell failure and one of causes of type 2 diabetes mellitus (T2DM). Although the KATP channel-dependent mechanism of GSIS has been broadly accepted for several decades, it does not fully describe the effects of glucose on insulin secretion. Emerging evidence has suggested that other mechanisms are involved. The present study demonstrated that geniposide enhanced GSIS in response to the stimulation of low or moderately high concentrations of glucose, and promoted glucose uptake and intracellular ATP levels in INS-1 cells. However, in the presence of a high concentration of glucose, geniposide exerted a contrary role on both GSIS and glucose uptake and metabolism. Furthermore, geniposide improved the impairment of GSIS in INS-1 cells challenged with a high concentration of glucose. Further experiments showed that geniposide modulated pyruvate carboxylase expression and the production of intermediates of glucose metabolism. The data collectively suggest that geniposide has potential to prevent or improve the impairment of insulin secretion in β-cells challenged with high concentrations of glucose, likely through pyruvate carboxylase mediated glucose metabolism in β-cells. © 2013 Liu et al. |
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| Publication date | 2013 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21269936 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 5dc68ed3-4220-4397-90d5-e49480f9bbe0 |
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| Record created | 2013-12-13 |
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| Record modified | 2021-09-17 |
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