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Hydrophilic interaction liquid chromatography-mass spectrometry for the analysis of paralytic shellfish poisoning (PSP) toxins

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.chroma.2005.05.056
AuthorSearch for: 1; Search for: 2; Search for: 3
Affiliation
  1. National Research Council of Canada
  2. National Research Council of Canada. NRC Institute for Marine Biosciences
  3. National Research Council of Canada. Measurement Science and Standards
FormatText, Article
Subjecthydrophilic interaction liquid chromatography; mass spectrometry; HILIC–MS; paralytic shellfish poisoning toxins; saxitoxins; marine toxins
Abstract
Hydrophilic interaction liquid chromatography (HILIC) was examined for the separation of paralytic shellfish poisoning (PSP) toxins using the stationary phase TSK-gel Amide-80®. The parameters tested included type of organic modifier and percentage in the mobile phase, buffer concentration, pH, flow rate and column temperature. Using mass spectrometric (MS) detection, the HILIC column allowed the determination of all the major PSP toxins in one 30 min analysis with a high degree of selectivity and sensitivity. The high percentage of organic modifier in the mobile phase and the omission of ion pairing reagents, both favored in HILIC, provided limits of detection (LOD) in the range 50–1000 nM in selected ion monitoring (SIM) mode on a single quadrupole LC–MS system. LOD in selected reaction monitoring (SRM) mode on a sensitive triple quadrupole system were as low as 5–30 nM. Excellent linearity of response was observed.
Publication date
Terms of use
  • Crown copyright © 2005 Published by Elsevier B.V.
In
  • Journal of Chromatography A 1081, no. 2 (22 July 2005): 190–201.
LanguageEnglish
Peer reviewedYes
NRC numberNRCC 42456
1404
NPARC number3538139
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Record identifier5dfa0bf8-2ed4-43f5-a3f8-b45dfbd044dd
Record created2009-03-01
Record modified2020-04-07
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