| Abstract | Cell division in soybean cells (Glycine max (L.) Merr.) grown as 100 ml batch cultures with a defined medium under continuous light has been partially synchronized by daily exchange of 25 of the cell suspension for an equal amount of fresh medium. Oscillations of the mitotic index showed 30 h periods, MI(max) = 25%. In cell suspensions maintained as a continuous culture partial synchrony was induced by flushing the cultures with Nā-pulses of 110 sec every 3–4 sec for 90 min per 24 and 30 h, MI(max) = 12–15%. When the suspensions were grown as a batch culture and N2-pulses administered 8 X 30min24h over a period of 4 days, cell division during the following period of constant aeration was synchronized for 1–2 cycles, MI(max) = 26% |
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