Studies on the chemical constitution of cell-wall lipopolysaccharides from Neisseria perflava

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1139/o68-176
Author	Search for: Adams, G. A.¹; Search for: Kates, M.¹; Search for: Shaw, D. H.¹; Search for: Yaguchi, M.¹
Affiliation	National Research Council of Canada
Format	Text, Article
Abstract	Lipopolysaccharides (LPS) were prepared from Neisseria perflava cells and separated into chloroform-soluble and chloroform-insoluble fractions in about equal proportions. The preparations were almost free from protein and contained only traces of peptide material. Both LPS fractions contained 3-deoxyoctulosonic acid (KDO), lipid A, glucose, rhamnose, heptose, glucosamine, galactosamine, ethanolamine, phosphate, and fatty acids. Both fractions had the same proportions of hexosamines and ethanolamine and the same fatty acid components (chiefly β-hydroxymyristic, β-faydroxylauric, octadecenoic, and palmitic acids), but differed markedly in their relative molar proportions of KDO to neutral sugars and of neutral sugars to total fatty acids. Equimolar proportions of hexosamine, ethanolamine, and phosphate in the lipid A moieties suggested that ethanolamine phosphate was linked to glucosamine, probably at the C-6 position. Fatty acids were bound in the LPS by both amide and ester linkages. Heptose units were linked linearly by 1 → 3 glycosidic bonds. About 25% of the glucose units were linked 1 → 3 in a chain; other glucose units were branched as were the rhamnose units. The proportions and arrangement of the heptose, KDO, and lipid A components suggest that the backbone part of the N. perflava lipopolysaccharide is very similar to that found in the Salmonella-Escherichia group.
Publication date	1968
Publisher	National Research Council of Canada. NRC Research Press
In	Canadian Journal of Biochemistry 46, no. 10: 1175–1184.
Note	The authors were affiliated with the National Research Council of Canada, Division of Biological Sciences when they authored this article.
Language	English
Peer reviewed	Yes
NRC number	NRCC 10275 ADAMS1968
NPARC number	9360240
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Record identifier	6381b9b4-8e5e-471a-9dd6-ccec45c09a24
Record created	2009-07-10
Record modified	2023-01-25

Date modified:: 2024-11-01