| DOI | Resolve DOI: https://doi.org/10.1016/j.carres.2013.11.006 |
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| Author | Search for: Lin, F.L.; Search for: Vinogradov, E.1; Search for: Deng, C.; Search for: Zeller, S.; Search for: Phelan, L.; Search for: Green, B.A.; Search for: Jansen, K.U.; Search for: Pavliak, V. |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | Capsular polysaccharides; Cross reactivity; Serogroup 33; Serotypes; Streptococcus pneumoniae; Structural assignments; Structure elucidation; Sugar residues; Acetylation; Polysaccharides; bacterial polysaccharide; epitope; galactose; glucose; monosaccharide; n acetylgalactosamine; phosphate; pneumococcal capsular polysaccharide; ribitol; ribitol phosphate; unclassified drug; acetylation; bacterial capsule; carbohydrate analysis; cross reaction; gas chromatography; heteronuclear single quantum coherence; linkage analysis; mass spectrometry; methylation; nuclear magnetic resonance spectroscopy; serotype; Streptococcus pneumoniae; Streptococcus pneumoniae |
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| Abstract | We report herein the previously unknown structures of the pneumococcal capsular polysaccharides serotype 33C and 33D, and a revised structure of serotype 33B. The syntenic pair 33B/33D has nearly identical polysaccharide repeat units with the exception of one sugar residue (→2-α-Glcp in 33B and →2-α-Galp in 33D). Serotype 33C is structurally more similar to 33B/33D than 33A/33F, in that it also possesses a backbone ribitol-phosphate group and a →3-β-GalpNAc residue, both of which are absent in the repeat units of 33A/33F. Serotype 33C is notably different from all other serogroup 33 polysaccharides, as there is no →3-β-Glcp residue and the location of the O-acetylation of the →5-β-Galf residue (O-6) differs from the other serogroup 33 polysaccharides (O-2). This completes the structural assignments of polysaccharides within serogroup 33 and provides a framework for understanding the recognition of epitopes by serogroup 33 typing sera based on observed cross-reactivities reported in the literature. © 2013 Elsevier Ltd. All rights reserved. |
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| Publication date | 2014 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21270855 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 67056423-5d4e-4d94-8359-2c836c7a16ee |
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| Record created | 2014-02-17 |
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| Record modified | 2020-04-22 |
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