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mSiglec-E, a novel mouse CD33-related siglec (sialic acid-binding immunoglobulin-like lectin) that recruits Src homology 2 (SH2)-domain-containing protein tyrosine phosphatases SHP-1 and SHP-2

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1042/0264-6021:3530483
AuthorSearch for: 1; Search for: 1; Search for: ; Search for: 1; Search for: 1
Affiliation
  1. National Research Council Canada. NRC Biotechnology Research Institute
FormatText, Article
Subjectpharmaceutical; glycosylation; inhibitory receptor; sialic acid; tyrosine phosphorylation
Abstract
The sialic acid-binding immunoglobulin-like lectins (siglecs) represent a recently defined distinct subset of the immunoglobulin superfamily. By using the Src homology 2 (SH2)-domain-containing protein tyrosine phosphatase SHP-1 as bait in a yeast two-hybrid screen, we have identified a new member of the mouse siglec family, mSiglec-E. The mSiglec-E cDNA encodes a protein of 467 amino acids that contains three extracellular immunoglobulin-like domains, a transmembrane region and a cytoplasmic tail bearing two immunoreceptor tyrosine-based inhibitory motifs (ITIMs). mSiglec-E is highly expressed in mouse spleen, a tissue rich in leucocytes. The ITIMs of mSiglec-E can recruit SHP-1 and SHP-2, two inhibitory regulators of immunoreceptor signal transduction. This suggests that the function of mSiglec-E is probably an involvement in haematopoietic cells and the immune system as an inhibitory receptor. When expressed in COS-7 cells, mSiglec-E was able to mediate sialic acid-dependent binding to human red blood cells, suggesting that mSiglec-E may function through cell-cell interactions. In comparison with the known members of the siglec family, mSiglec-E exhibits a high degree of sequence similarity to both human siglec-7 and siglec-9. The gene encoding mSiglec-E is localized in the same chromosome as that encoding mouse CD33. Phylogenetic analysis reveals that neither mouse mSiglec-E nor CD33 shows a clear relationship with any human siglecs so far identifed.
Publication date
PublisherPortland Press
Biochemical Society
In
  • Biochemical Journal 353, no. 3 (1 February 2001): 483–492.
LanguageEnglish
NRC number43002
46150
NPARC number3538765
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Record identifier6b1f6533-bfa6-47e3-a856-e0d35a8ad641
Record created2009-03-01
Record modified2024-09-17
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