| DOI | Resolve DOI: https://doi.org/10.1007/BF00274010 |
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| Author | Search for: Herzog, Albert; Search for: Yaguchi, Makoto1; Search for: Cabezon, Teresa; Search for: Corchuelo, Maria-Clara; Search for: Petre, Jean; Search for: Bollen, Alex |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Subject | gene cluster; phenylalanine; missense mutation; ribosomal protein; Escherichia coli |
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| Abstract | The conditionally lethal mutation, 286lmis, has been mapped inside the ribosomal protein gene cluster at 72 minutes on the Escherichia coli chromosome and was found to cotransduce at 97% with rpsE (S5). The 2861mis mutation leads to thermosensitivity and impaired assembly in vivo of 30S ribosomal particles at 42°C. The strain carrying the mutation has an altered S17 ribosomal protein; the mutational alteration involves a replacement of serine by phenylalanine in protein S17. Spontaneous reversion to temperature independence can restore the normal assembly in vivo of 30S ribosomal subunits at 42°C and the normal chromatographical sehaviour of the S17 ribosomal protein in vitro. We conclude therefore that the 2861mis mutation affects the structural gene for protein S17 (rpsQ). |
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| Publication date | 1979-01 |
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| Publisher | Springer |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | HERZOG1979 |
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| NPARC number | 9376282 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 6c756b85-eaca-416d-a473-1785a2dfbbf6 |
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| Record created | 2009-07-10 |
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| Record modified | 2022-11-18 |
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