| Abstract | The positional distribution of fatty acidsin the sulfolipids from five different plant specieswas analyzed by using a lipase from-the fungusRhizopus delemar. Palmitic acid, which is a majorcomponent of all the sulfolipids, was esterified pre-ferentially at C-2 of glycerol. Gas-liquid chromato-graphy of trimethylsilyl ethers of hydrogenatedsulfolipids (sulfonic acid as methyl ester) showedthe distribution of various possible species resultingfrom pairing of fatty acids. In all sulfolipids in-vestigated the major species was l'-0-stearoyl-2'-0-palmitoyl-3'-0-(6-deoxy-6-sulfo-α-D-glucopyra-nosyl)-sn-glycerol. In contrast to the galactolipids,dipalmitoyl species were also present. Nuclearmagnetic resonance spectra of the triacetates ofsulfolipid methyl esters from 4 different plant speciesconfirmed that all had the same structure and hadthe gluco-configuration. In addition, positionaldistribution of fatty acids in several digalactosyldiacylglycerols was investigated. In these lipidspalmitic acid does not show a specific distribution. The present findings and other data demonstratethat in higher plants the main species of the variouspolar lipids have different diacylglycerol moieties |
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