The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1105/tpc.12.2.279
Author	Search for: Despres, Charles¹; Search for: Delong, Catherine¹; Search for: Glaze, Sarah.¹; Search for: Liu, Enwu¹; Search for: Fobert, P. (Pierre)¹
Affiliation	National Research Council Canada. NRC Plant Biotechnology Institute
Format	Text, Article
Abstract	The Arabidopsis NPR1 gene is essential in activating systemic, inducible plant defense responses. To gain a better understanding of NPR1 function, we conducted a yeast two-hybrid screening procedure and identified a differential interaction between NPR1 and all known members of the Arabidopsis TGA family of basic leucine zipper transcription factors. In the electrophoretic mobility shift assay, NPR1 substantially increased the binding of TGA2 to its cognate promoter element (as-1) as well as to a positive salicylic acid–inducible element (LS7) and a negative element (LS5) in the promoter of the pathogenesis-related PR-1 gene. Proteins encoded by npr1 mutants interacted poorly with TGA2 and did not substantially increase TGA2 binding to the as-1, LS5, or LS7 elements, thus establishing a link between the loss of disease resistance and the loss of TGA2 interaction and NPR1-enhanced DNA binding. Coupled with observations that the DNA binding activity of TGA factors is deregulated in npr1 plants, the results suggest that NPR1-mediated DNA binding of TGA2 is critical for activation of defense genes.
Publication date	2000-02
Publisher	Oxford Academic American Society of Plant Biologists
In	Plant Cell 12, no. 2 (February 2000): 279–290.
Language	English
Peer reviewed	Yes
NPARC number	13053406
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Record identifier	6f924108-5385-43a0-8638-bfa0548fbd5d
Record created	2009-11-25
Record modified	2023-04-14

Date modified:: 2025-12-09