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Identification of unnatural phaseic acid as a metabolite derived from exogenously added (−)-abscisic acid in a maize cell suspension culture

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/S0031-9422(00)89790-3
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for:
Affiliation
  1. National Research Council Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectgramineae; cell culture; metabolism; abscisic acid; phaseic acid; chiral gas; chromatography
Abstract
Unnatural phaseic acid (U-PA) was identified by chiral GC as a minor component of the metabolite mixture obtained from the medium of a maize cell suspension culture treated with unnatural (−)(R)-abscisic acid (U-ABA). When U-[3′,5′,5′,7′,7′,7′]-hexadeutero-ABA was fed to the cell suspension, d₆-PA was obtained, establishing its origin from the exogenously-added U-ABA. The major metabolise produced was identified as U-7′-hydroxyabscisic acid (U-7′-HOABA [d₅-7′-HOABA from d₆-U-ABA]. Confirmation of the assignments was obtained by HPLC/LC/CFSIMS analysis of the metabolise mixtures. This result corroborates a previous report of the formation of U-PA from racemic ABA in avocado mesocarp tissue.
Publication date
PublisherElsevier
In
  • Phytochemistry 36, no. 3 (June 1994): 647–650.
LanguageEnglish
Peer reviewedYes
IdentifierS0031942200897903
NRCC-37340
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Record identifier714296eb-7e38-45b8-805d-c901087f51c2
Record created2023-10-25
Record modified2023-10-25
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