| Download | - View accepted manuscript: Detection of azaspiracids in mussels using electrochemical immunosensors for fast screening in monitoring programs (PDF, 5.0 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1016/j.snb.2018.02.046 |
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| Author | Search for: Leonardo, Sandra; Search for: Kilcoyne, Jane; Search for: Samdal, Ingunn A.; Search for: Miles, Christopher O.1; Search for: O’sullivan, Ciara K.; Search for: Diogène, Jorge; Search for: Campàs, Mònica |
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| Affiliation | - National Research Council of Canada. Measurement Science and Standards
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| Format | Text, Article |
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| Subject | azaspiracid; antibody; electrochemical immunosensor; protein G; biotin; mussel |
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| Abstract | Given the widespread occurrence of azaspiracids (AZAs), it is clearly necessary to advance in simple and low-cost methods for the rapid detection of these marine toxins in order to protect seafood consumers. To address this need, electrochemical immunosensors for the detection of AZAs based on a competitive direct immunoassay using peroxidase-labelled AZA as a tracer were developed. An anti-AZA polyclonal antibody was immobilised in a controlled and stable manner on protein G or avidin-coated electrodes. Experimental conditions were first optimised using colorimetric immunoassays on microtitre plates, providing intermediate products already applicable to the accurate detection of AZAs. Then, transfer of the protein G and avidin–biotin interaction-based immunoassays to 8-electrode arrays provided compact and miniaturised devices for the high-throughput detection of AZAs. The low amounts of immunoreagents required as well as the potential for reusability of the avidin–biotin interaction-based immunosensors represented significant economic savings as well as a contribution to sustainability. The electrochemical immunosensors enabled the quantification of all regulated AZAs below the regulatory limit, as well as a broad range of other toxic AZA analogues (from 63 ± 3 to 2841 ± 247 μg AZA-1 equiv./kg for the protein G-based immunosensor and from 46 ± 2 to 3079 ± 358 μg AZA-1 equiv./kg for the avidin–biotin interaction-based immunosensor). The good agreement between the results obtained by the immunosensors and LC–MS/MS in the analysis of naturally contaminated mussel samples demonstrated the easy implementation of electrochemical immunosensors for routine analysis of AZAs in food safety monitoring programs. |
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| Publication date | 2018-02-06 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23003585 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 71b3d66c-81a1-4d44-bfc5-74b842e4d41e |
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| Record created | 2018-07-20 |
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| Record modified | 2020-06-03 |
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