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Sequence determination for N-linked oligosaccharides through the use of the nuclear Overhauser effect

From National Research Council Canada

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Subjectalpha; carbohydrates; carbohydrate conformation; carbohydrate sequence; chains; chemical methods; elucidation; GlcNAc; glycopeptide; glycopeptides; magnetic resonance spectroscopy; NMR spectroscopy; methods; N-linked; nuclear; nuclear magnetic resonance; oligosaccharides; Overhauser; residues; resonance; sequences; technique
Abstract
A nuclear magnetic resonance technique, the nuclear Overhauser effect, has been used to confirm the presence of Manα1-2, GlcNAcβ1-2, and Manα1-3 residues and to establish the branching pattern and sequences in four classes of N-linked oligosaccharides. This method offers a rapid and nondestructive approach to the elucidation of sequences in carbohydrate chains, in contrast to enzymatic and chemical methods
Publication date
PublisherAmerican Society for Biochemistry and Molecular Biology
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  • The Journal of Biological Chemistry 258, no. 3: 1431–1434.
LanguageEnglish
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IdentifierBRISSON1983H
NPARC number9366983
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Record identifier725aa6f4-dc22-4c9e-99ea-3e63fe5429e4
Record created2009-07-10
Record modified2020-05-27
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