| DOI | Resolve DOI: https://doi.org/10.1016/0304-4211(79)90087-7 |
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| Author | Search for: Kartha, K. K.1; Search for: Leung, N. L.1; Search for: Gamborg, O. L.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Abstract | A procedure has been developed for the freeze-preservation of pea (Pisum sativum L.) meristems and subsequent plant regeneration. The meristems were precultured in the presence of 5% DMSO for 48 h followed by freezing at cooling velocities varying from 0.5 to 1.0°C/min up to a terminal freezing temperature of −40°C and subsequently stored in liquid nitrogen (−196°C). Maximum survival as determined by the regrowth of meristems into whole plants was obtained at a cooling rate of 0.6°C/min after 1 h storage in liquid nitrogen (73%). The meristems were in storage in liquid nitrogen for up to 26 weeks and upon reculture more than 60% of the meristems differentiated into whole plants. Cold conditioning of meristems at 4°C prior to freezing without preculture in the presence of DMSO resulted in lower survival rates. Glycerol and ethylene glycol were ineffective cryoprotectants. Ultra-rapid freezing of meristems by direct immersion in liquid nitrogen was also lethal. |
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| Publication date | 1979-05 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | 0304421179900877 |
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| NRC number | NRCC-17111 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 73eb6844-8814-4d31-a42a-47885f95abad |
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| Record created | 2023-07-06 |
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| Record modified | 2023-07-11 |
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