| DOI | Resolve DOI: https://doi.org/10.1039/c2an35128e |
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| Author | Search for: Zheng, D.; Search for: Vashist, S.K.; Search for: Al-Rubeaan, K.; Search for: Luong, J.H.T.1; Search for: Sheu, F.-S. |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | carbon; glass; glucose; glucose oxidase; immobilized enzyme; Aspergillus niger; chemistry; electrochemistry; electrode; enzymology; genetic procedures; metabolism; methodology; time; Aspergillus niger; Biosensing Techniques; Carbon; Electrochemistry; Electrodes; Enzymes, Immobilized; Glass; Glucose; Glucose Oxidase; Time Factors |
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| Abstract | A rapid and simple procedure was developed for the preparation of a highly stable and leach-proof glucose oxidase (GOx)-bound glassy carbon electrode (GCE). Crosslinked GOx via glutaraldehyde was drop-cast on a KOH-pretreated GCE followed by drop-casting of 3-aminopropyltriethoxysilane (APTES) to form a stable bioactive layer. At -0.45 V, the biosensor exhibited a wide dynamic detection range of 0.5-48 mM for commercial glucose and 1.3-28.2 mM for Sugar-Chex blood glucose linearity standards. Several endogenous electroactive substances and drug metabolites commonly found in blood were tested and provoked no signal response. To our knowledge, the developed procedure is the most rapid method for preparing a glucose biosensor. The biosensor suffered no biofouling after 7 days of immersion in Sugar-Chex blood glucose. With excellent production reproducibility, GOx-bound electrodes stored dry at room temperature retained their initial activity after several weeks. © 2012 The Royal Society of Chemistry. |
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| Publication date | 2012 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21269213 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 749bbafd-4f02-4b1d-924d-68487e96c7f1 |
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| Record created | 2013-12-12 |
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| Record modified | 2020-04-21 |
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