National Research Council of Canada. NRC Biotechnology Research Institute
In the present study, we used previous termmicrowavenext term energy instead of conventional heating to transform poly-3-hydroxybutyrate (PHB) into methyl 3-hydroxybutyrate (Me-3HB) in acidified methanol (H2SO4, 10%, v/v) mixture in less than 4 min at 10% previous termmicrowavenext term power. The previous termmicrowavenext term assisted method was then applied to analyze PHB produced by Alcaligenes latus. The PHB content in the biomass determined using previous termmicrowavenext term heating was comparable to the amount found by conventional heating. Moreover, the new esterification method was at least 50 times faster than the conventional method, affording a significant saving of time and energy.
Journal of Chromatography A1154, no. 1-2: 473–476.