Hepatoprotective effect of Combretum quadrangulare and its constituents

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1248/bpb.23.456
Author	Search for: Banskota, Arjun Hari; Search for: Tezuka, Yasuhiro; Search for: Adnyana, I Ketut; Search for: Xiong, Quanbo; Search for: Hase, Koji; Search for: Tran, Kim Qui; Search for: Tanak, Ken; Search for: Saiki, Ikuo; Search for: Kadota, Shigetoshi
Format	Text, Article
Subject	Combretum quadrangulare; hepatoprotective effect; flavone, cycloartane-type triterpene; flavone C-glycoside
Abstract	The MeOH extract of leaves of Combretum quadrangulare showed significant hepatoprotective effect on D-galactosamin (D-GalN)/lipopolysaccharide (LPS)induced experimental liver injury in mice and on D-GalN/tumor necrosis factor-α (TNF-α)-induced cell death in primary cultured mouse hepatocytes. Phytochemical investigation led to the isolation of thirty cycloartane-type triterpenes together with betulinic acid, β-stitosterol, β-sitosterol glucoside, 4 flavones (34-37), and 3 flavone C-glucosides (38-40). These compounds showed various potencies of hepatoprotective effect on D-GalN/TNF-α-induced cell death in primary cultured mouse hepatocytes. Quadrangularol B (29), methyl quadrangularate I (33), kamatakenin (34), 5, 7, 4'-trihydroxy-3, 3'-dimethoxyflavone (35), 5, 4'-dihydroxy-3, 7, 3'-trimethoxyflavone (36) and isokaempferide (37) showed strong inhibitory effect on TNF-α-induced cell death with IC50 values of 34.3, 33.7, 13.3, 22.4, 13.4 and 22.8 μM, respectively, whereas clinically-used silibinin had an IC50 value of 39.6 μM and glycyrrhizin showed very weak inhibitory effect. Methyl quadarangularates A (30) and N (32), norquadrangularic acid B (31) and vitexin (40) also showed potent inhibition on TNF-α-induced cell death with IC50 values of 45.7, 89.3, 67.6 and 40.1 μM, respectively. The flavonoids and some of the cycloartane-type triterpenes appeared to be the hepatoprotective priniciples of the leaves of C. quadrangulare.
Publication date	2000
In	Biological and Pharmaceutical Bulletin 23, no. 4 (2000): 456–460.
Language	English
Peer reviewed	Yes
NRC publication	This is a non-NRC publication "Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.
NPARC number	21275301
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Record identifier	79d12ea4-384c-4443-8121-58d577a6b103
Record created	2015-06-04
Record modified	2020-03-26

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