| DOI | Resolve DOI: https://doi.org/10.1088/0957-4484/23/20/205101 |
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| Author | Search for: Gu, Q.1; Search for: Xing, J.Z.; Search for: Huang, M.; Search for: He, C.; Search for: Chen, J.1 |
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| Affiliation | - National Research Council of Canada. National Institute for Nanotechnology
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| Format | Text, Article |
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| Subject | Aqueous suspensions; Cancer cells; Cancer therapy; Caprolactone; Drug loading; Drug release; Encapsulation efficiency; Fetal bovine serum; Hydration method; In-vitro; Inhibitory concentration; Low toxicity; Lung cells; Modified film; Phosphate-buffered salines; Pluronics; Polymeric micelle; Propylene glycols; Transmission electron microscopy (TEM); Atomic force microscopy; Dynamic light scattering; Encapsulation; Loading; Nanoparticles; Transmission electron microscopy; Polyethylene glycols; antineoplastic agent; camptothecin; drug derivative; irinotecan; nanocapsule; apoptosis; cell survival; chemistry; diffusion; drug effect; experimental neoplasm; metabolism; micelle; tumor cell culture; Antineoplastic Agents, Phytogenic; Apoptosis; Camptothecin; Cell Survival; Diffusion; Micelles; Nanocapsules; Neoplasms, Experimental; Tumor Cells, Cultured |
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| Abstract | 7-Ethyl-10-hydroxycamptothecin (SN-38) loaded poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (Pluronic F-108) and poly(ethylene glycol)-block-poly("-caprolactone) (PEG-b-PCL) nanoparticles were successfully prepared by a modified film hydration method and characterized by scanning electric microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM) and dynamic light scattering (DLS). Satisfactory drug loading of 20.73 ± 0.66% and a high encapsulation efficiency of 83:83 ± 1:32% were achieved. The SN-38 nanoparticles (SN-38 NPs) can completely disperse into a phosphate buffered saline (PBS) medium to produce a clear aqueous suspension that remains stable for up to three days. Total drug releases were 67.91% and 91.09% after 24 h in a PBS or fetal bovine serum (FBS) medium. Half maximal inhibitory concentration (IC50) tests of SN-38 and SN-38 NPs on A549 lung cells produced results of 200.0±14.9 ng ml -1 and 80.0±4.6 ng ml -1, respectively. Similarly, IC50 tests of SN-38 and SN-38 NPs on MCF-7 breast cells yielded results of 16.0 ± 0.7 ng ml -1 and 8.0 ± 0.5 ng ml -1, respectively. These in vitro IC 50 studies show significant (p < 0.01) enhancement of the SN-38 NP drug efficiency in killing cancer cells in comparison to the free drug SN-38 control. All the materials used for this nanoformulation are approved by the US FDA, with the virtue of extremely low toxicity to normal cells. © 2012 IOP Publishing Ltd. |
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| Publication date | 2012 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21269485 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 7a32c2f4-ed33-4747-b065-75df8c14a016 |
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| Record created | 2013-12-12 |
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| Record modified | 2020-04-21 |
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