| Download | - View accepted manuscript: Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada) (PDF, 1.6 MiB)
- View supplementary information: Hydrocarbon biodegradation by Arctic sea-ice and sub-ice microbial communities during microcosm experiments, Northwest Passage (Nunavut, Canada) (ZIP, 99 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1093/femsec/fiw130 |
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| Author | Search for: Garneau, Marie-Ève; Search for: Michel, Christine; Search for: Meisterhans, Guillaume1; Search for: Fortin, Nathalie1; Search for: King, Thomas L.; Search for: Greer, Charles W.1; Search for: Lee, Kenneth |
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| Affiliation | - National Research Council of Canada. Energy, Mining and Environment
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| Format | Text, Article |
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| Subject | arctic; sea ice; hydrocarbon biodegradation; bioremediation; 16S ribosomal RNA gene sequencing; microbial community fingerprinting |
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| Abstract | The increasing accessibility to navigation and offshore oil exploration brings risks of hydrocarbon releases in Arctic waters. Bioremediation of hydrocarbons is a promising mitigation strategy but challenges remain, particularly due to low microbial metabolic rates in cold, ice-covered seas. Hydrocarbon degradation potential of ice-associated microbes collected from the Northwest Passage was investigated. Microcosm incubations were run for 15 days at -1.7°C with and without oil to determine the effects of hydrocarbon exposure on microbial abundance, diversity and activity, and to estimate component-specific hydrocarbon loss. Diversity was assessed with automated ribosomal intergenic spacer analysis and ion torrent 16S rRNA gene sequencing. Bacterial activity was measured by ³H-leucine uptake rates. After incubation, sub-ice and sea-ice communities degraded 94% and 48% of the initial hydrocarbons, respectively. Hydrocarbon exposure changed the composition of sea-ice and sub-ice communities; in sea-ice microcosms, Bacteroidetes (mainly Polaribacter) dominated whereas in sub-ice microcosms, Epsilonproteobacteria contribution increased, but that of Alphaproteobacteria and Bacteroidetes decreased. Sequencing data revealed a decline in diversity and increases in Colwellia and Moritella in oil-treated microcosms. Low concentration of dissolved organic matter (DOM) in sub-ice seawater may explain higher hydrocarbon degradation when compared to sea ice, where DOM was abundant and composed of labile exopolysaccharides. |
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| Publication date | 2016-07-06 |
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| Publisher | Oxford University Press
Federation of European Microbiological Societies |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23000460 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 7c0aa52f-edde-4536-bc0b-f3df34a692ee |
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| Record created | 2016-07-20 |
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| Record modified | 2024-02-05 |
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