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Characterization of the lipopolysaccharide O-antigen of Cronobacter turicensis HPB3287 as a polysaccharide containing a 5,7-diacetamido-3,5,7,9- tetradeoxy-d-glycero-d-galacto-non-2-ulosonic acid (legionaminic acid) residue

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.carres.2011.09.003
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Affiliation
  1. National Research Council of Canada. NRC Institute for Biological Sciences
FormatText, Article
SubjectBacterial polysaccharides; Chemical and physical analysis; Composition analysis; Cronobacter turicensis; D-galactose; D-glucose; Enterobacter; Food-borne pathogens; High molecular mass; High resolution; Lipopolysaccharides; Molar ratio; O-Antigen; O-polysaccharide; Pentasaccharides; Alkylation; Chemical analysis; Glucose; Mass spectrometry; Resonance; Antigens; bacterial polysaccharide; bacterium lipopolysaccharide; O antigen; carbohydrate analysis; Cronobacter turicensis; Enterobacter; mass spectrometry; molecular weight; nuclear magnetic resonance spectroscopy; structure analysis; Carbohydrate Sequence; Cronobacter sakazakii; Hydrolysis; Lipopolysaccharides; Magnetic Resonance Spectroscopy; Mass Spectrometry; Methylation; Molecular Sequence Data; O Antigens; Oxidation-Reduction; Sialic Acids; Bacteria (microorganisms); Enterobacter sakazakii; Negibacteria
Abstract
Cronobacter turicensis, previously known as Enterobacter sakazakii, is a Gram-negative opportunistic food-borne pathogen that has been reported as a cause of life-threatening neonatal infections. From chemical and physical analyses involving composition analysis, methylation, two-dimensional high-resolution nuclear magnetic resonance, and mass spectrometry methods, the antigenic O-polysaccharide in the smooth-type lipopolysaccharide of C. turicensis (strain HPB 3287) was determined to be a high molecular mass polymer of a repeating pentasaccharide unit composed of d-galactose, d-glucose, 2-acetamido-2-deoxy-d-galactose, and 5,7-diacetamido-3,5,7,9-tetradeoxy-d- glycero-d-galacto-non-2-ulosonic acid (legionaminic acid), in a molar ratio 2:1:1:1, and having the structure. © 2011 Elsevier Ltd. All rights reserved.
Publication date
In
  • Carbohydrate Research 346, no. 16 (2011): 2589–2594.
LanguageEnglish
Peer reviewedYes
NPARC number21271342
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Record created2014-03-24
Record modified2020-04-21
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