| DOI | Resolve DOI: https://doi.org/10.1105/tpc.114.124743 |
|---|
| Author | Search for: Borg, M.; Search for: Rutley, N.; Search for: Kagale, S.1; Search for: Hamamura, Y.; Search for: Gherghinoiu, M.; Search for: Kumar, S.; Search for: Sari, U.; Search for: Esparza-Franco, M.A.; Search for: Sakamoto, W.; Search for: Rozwadowski, K.; Search for: Higashiyama, T.; Search for: Twell, D. |
|---|
| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
|
|---|
| Format | Text, Article |
|---|
| Abstract | The production of the sperm cells in angiosperms requires coordination of cell division and cell differentiation. In Arabidopsis thaliana, the germline-specific MYB protein DUO1 integrates these processes, but the regulatory hierarchy in which DUO1 functions is unknown. Here, we identify an essential role for two germline-specific DUO1 target genes, DAZ1 and DAZ2, which encode EAR motif-containing C2H2-type zinc finger proteins. We show that DAZ1/DAZ2 are required for germ cell division and for the proper accumulation of mitotic cyclins. Importantly, DAZ1/DAZ2 are sufficient to promote G2- to M-phase transition and germ cell division in the absence of DUO1. DAZ1/DAZ2 are also required for DUO1-dependent cell differentiation and are essential for gamete fusion at fertilization. We demonstrate that the two EAR motifs in DAZ1/DAZ2 mediate their function in the male germline and are required for transcriptional repression and for physical interaction with the corepressor TOPLESS. Our findings uncover an essential module in a regulatory hierarchy that drives mitotic transition in male germ cells and implicates gene repression pathways in sperm cell formation and fertility. |
|---|
| Publication date | 2014-05 |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NRC number | NRC-ACRD-56070 |
|---|
| NPARC number | 21272400 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 82d28fab-facc-424a-b1b2-1c986cef320d |
|---|
| Record created | 2014-11-25 |
|---|
| Record modified | 2020-04-22 |
|---|
