| DOI | Resolve DOI: https://doi.org/10.1128/IAI.69.9.5375-5384.2001 |
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| Author | Search for: Kaplan, Jeffrey B.; Search for: Perry, Malcolm B.1; Search for: Maclean, Leann L.1; Search for: Furgang, David; Search for: Wilson, Mark E.; Search for: Fine, Daniel H. |
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| Editor | Search for: Moore, R. N. |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Subject | aggregatibacter actinomycetemcomitans; O antigens; periodontitis |
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| Abstract | The oral bacterium Actinobacillus actinomycetemcomitans is implicated as a causative agent of localized juvenile periodontitis (LJP). A. actinomycetemcomitans is classified into five serotypes (a to e) corresponding to five structurally and antigenically distinct O polysaccharide (O-PS) components of their respective lipopolysaccharide molecules. Serotype b has been reported to be the dominant serotype isolated from LJP patients. We determined the lipopolysaccharide O-PS structure from A. actinomycetemcomitans CU1000, a strain isolated from a 13-year-old African-American female with LJP which had previously been classified as serotype b. The O-PS of strain CU1000 consisted of a trisaccharide repeating unit composed ofl-rhamnose and 2-acetamido-2-deoxy-d-galactose (molar ratio, 2:1) with the structure →2)-α-l-Rhap-(1–3)-2-O-(β-d-GalpNAc)-α-l-Rhap-(1→. O-PS from strain CU1000 was structurally and antigenically distinct from the O-PS molecules of the five known A. actinomycetemcomitans serotypes. Strain CU1000 was mutagenized with transposon IS903φkan, and three mutants that were deficient in O-PS synthesis were isolated. All three transposon insertions mapped to a single 1-kb region on the chromosome. The DNA sequence of a 13.1-kb region surrounding these transposon insertions contained a cluster of 14 open reading frames that was homologous to gene clusters responsible for the synthesis of A. actinomycetemcomitans serotype b, c, and e O-PS antigens. The CU1000 gene cluster contained two genes that were not present in serotype-specific O-PS antigen clusters of the other five knownA. actinomycetemcomitans serotypes. These data indicate that strain CU1000 should be assigned to a new A. actinomycetemcomitans serotype, designated serotype f. A PCR assay using serotype-specific PCR primers showed that 3 out of 20 LJP patients surveyed (15%) harbored A. actinomycetemcomitans strains carrying the serotype f gene cluster. The finding of an A. actinomycetemcomitansserotype showing serological cross-reactivity with anti-serotype b-specific antiserum suggests that a reevaluation of strains previously classified as serotype b may be warranted. |
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| Publication date | 2001-09-01 |
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| Publisher | American Society for Microbiology |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 9373976 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 83cf042d-e0df-4df6-8a6d-4481bf840f02 |
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| Record created | 2009-07-10 |
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| Record modified | 2021-04-27 |
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