Identification of genes differently expressed in Atlantic salmon (Salmo salar) in response to infection by Aermonas salmonicida using cDNA microarray technology

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.dci.2004.08.004
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Name affiliation
  1. National Research Council of Canada. Aquatic and Crop Resource Development
  2. National Research Council of Canada. NRC Institute for Marine Biosciences
  3. National Research Council of Canada. Human Health Therapeutics
FormatText, Article
SubjectAeromonas salmonicida; atlantic salmon; furunculosis; gene expression; innate immunity; microarray; Salmo salar
Abstract
The response of Atlantic salmon, Salmo salar, to infection by the bacterial pathogen Aeromonas salmonicida (the causative agent of furunculosis), was investigated using a cohabitation model and a custom Atlantic salmon cDNA microarray consisting of over 4000 different amplicons. Pooled samples of each of three immune-relevant tissues (spleen, head kidney and liver) were obtained from fish exposed to infected salmon for 13 days. Reverse transcription-PCR assays were used to verify the differential expression of 12 candidate genes uncovered by microarray analysis. Among the differentially expressed genes were several previously revealed by suppression subtractive hybridization and EST surveys and that are recognized to encode humoral components of the innate immune system. Other genes identified in this study were not previously associated with infection. In addition, a number of genes with no known homologs were uncovered. Determination of their specific roles during infection may lead to a better understanding of innate immunity.
Publication date
Terms of use
  • Crown copyright © 2004 Published by Elsevier Ltd.
In
  • Developmental & Comparative Immunology 29, no. 4 (2005): 333–347.
LanguageEnglish
Peer reviewedYes
NRC numberNRCC 42446
1411
NPARC number3538500
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