| Abstract | Anthers of two barley (Hordeum vulgare L.) cultivars, Bonanza and Elrose were cultured in media A or K1 with different levels of 2,4-D and zeatin riboside (Z-r). The results indicated that a combination of 2,4-D and Z-r in their optimal ranges (0.25–1.0 mg/L and 0.125–0.25 mg/L, respectively) significantly increased pollen callus induction in Bonanza anthers. Addition of 2,4-D alone (optimal range 0.25–2.0 mg/L) increased the frequency of callus formation in Elrose, while Z-r (0.125–2.0 mg/L) had no obvious effect on callus induction. When Z-r concentration was higher than 2.0 mg/L it decreased callus formation. At high concentrations (2.0–4.0 mg/L) 2,4-D alone induced loose calli which were not able to develop into plantlets. |
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