| Download | - View accepted manuscript: Enhancing the Outcome of Microarray-Based Molecular Diagnostics (PDF, 847 KiB)
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| Author | Search for: Tulpan, Dan; Search for: Léger, Serge |
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| Format | Text, Article |
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| Conference | The Advances in Microarray Technology (AMT), May 7-8, 2008, Barcelona, Spain |
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| Abstract | Molecular diagnostics determines how genes and proteins are interacting in a cell and focuses upon gene and protein activity patterns in different types of cancerous or precancerous cells. Molecular diagnostics uncovers these sets of changes and captures this information as expression patterns via various data analysis techniques. The accuracy of the diagnostic process is hampered by many factors like: quality of the biological samples, accuracy of analytical methods and quality of microarray probes.While the first two factors can be addressed by using better sample storage procedures and careful sample manipulation and by carefully choosing analytical techniques with wider domains of applicability and proven higher accuracies, the third factor is most of the time hard to adjust, given the rigidity of existing microarray platforms and increased costs for their re-design and re-validation. Here, it is presented a technique that allows researchers to use experimental data (expression values) obtained with existing microarray platforms whose probe sets may not match the biological information known today. The technique is capable of adjusting the expression values in the experimental data by spotting inconsistencies between probes and their sequence origins and estimating their level of hybridization using molecular thermodynamic modeling. |
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| Publication date | 2008 |
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| In | |
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| Language | English |
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| NRC number | NRCC 50359 |
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| NPARC number | 8913778 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 8a5624cf-07a3-44eb-a42e-ef738c603bcf |
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| Record created | 2009-04-22 |
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| Record modified | 2020-08-12 |
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