Determination of chemical purity and isotopic composition of natural and carbon-13-labeled arsenobetaine bromide standards by quantitative 1H-NMR

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/s00216-016-9827-y
AuthorSearch for: 1; Search for: 2; Search for: 1; Search for: 1ORCID identifier: https://orcid.org/0000-0002-2377-2615; Search for: 3; Search for: 1; Search for: 1ORCID identifier: https://orcid.org/0000-0002-3349-5535
Name affiliation
  1. National Research Council Canada. Measurement Science and Standards
  2. National Research Council Canada. Security and Disruptive Technologies
  3. National Research Council Canada. Energy, Mining and Environment
FormatText
TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
ISSN1618-2642
1618-2650
Volume49
Issue8
Pages681–691
Subjectisotope ratio measurements; arsenobetaine; standard; certified reference material
Abstract
In this study, we report the characterization of three arsenobetaine-certified reference materials by quantitative NMR. We have synthesized an arsenobetaine bromide high-purity standard of natural isotopic composition (ABET-1) and two carbon-13-labeled isotopic standards (BBET-1 and CBET-1). Assignments of the chemical purity and isotopic composition are not trivial in the case of arsenobetaine, and in this study we utilized quantitative 1H-NMR techniques for the determination of the mass fractions (chemical purity). The isotopic purity of all three standards was also assessed by NMR from the carbon-13 satellite signals. The standards are non-hygroscopic, highpurity (ca. 0.99 g/g), and the carbon-13 enrichment for both isotopic standards is x(13C) ≈ 0.99. These standards are designed for use as primary calibrators for mass spectrometric determination of arsenobetaine in environmental samples.
Publication date
PublisherSpringer
LanguageEnglish
Peer reviewedYes
NPARC number23000646
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