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In vitro growth responses and plant regeneration from cryopreserved meristems of cassava (Manihot esculenta crantz)

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/S0044-328X(82)80099-8
AuthorSearch for: 1; Search for: 1; Search for:
Affiliation
  1. National Research Council of Canada. Prairie Regional Laboratory
FormatText, Article
SubjectManihot esculenta; cryopreservation; droplet-freezing method; dimethylsulfoxide (DMSO); plant regeneration in vitro; germplasm preservation
Abstract
A droplet-freezing method has been developed for the cryopreservation of cassava, Manihot esculenta Crantz, meristems. The meristems treated with a 15 % DMSO + 3% sucrose solution for 15 min were frozen over an 18 μm aluminium foil in 2–3 μl droplets of the cryoprotectants in plastic petri dishes at a cooling rate of 0.5°C/min to various sub-zero temperatures (-20; -25; -30 and -40°C) and stored in liquid nitrogen (-196°C). The meristems retrieved from liquid nitrogen storage, upon thawing and return to in vitro culture on plant regeneration medium, exhibited various morphogenetic responses such as differentiation of callus and leaves and whole plantlets. The plantlets were successfully grown in pots.
Publication date
PublisherElsevier
In
  • Zeitschrift für Pflanzenphysiologie 107, no. 2 (August 1982): 133–140.
LanguageEnglish
Peer reviewedYes
IdentifierS0044328X82800998
NRCC-20411
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Record identifier8fba8bd7-1744-42c8-b63a-1c1d5b484363
Record created2023-11-29
Record modified2023-11-29
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