A ¹H-NMR method for determining temperature in cell culture perfusion systems

DOI	Resolve DOI: https://doi.org/10.1002/mrm.1910290120
Author	Search for: Lutz, N. W.; Search for: Kuesel, A. C.; Search for: Hull, W. E.
Format	Text, Article
Subject	1H-NMR; temperature; cell culture; perfusion
Abstract	This report describes a noninvasive 1H-NMR method for measuring absolute temperatures (±0.2°C) in biological samples and, in particular, in cell culture perfusion systems, utilizing the linear temperature dependence of the water chemical shift relative to the temperature-independent shift of one of the components of the biological medium, e.g., pyruvate, acetate, or lactate. The effects of flow on temperature can be monitored and appropriate adjustments of the temperature controller can be made.
Publication date	1993
In	Magnetic Resonance in Medicine 29, no. 1 (January 1993): 113–118.
Language	English
Peer reviewed	Yes
NRC publication	This is a non-NRC publication "Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.
NRC number	377
NPARC number	9742172
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Record identifier	9090a8f6-5231-41a9-8abd-5d17b45b6514
Record created	2009-07-17
Record modified	2020-04-24